Transcript analysis and expression profiling of three heat shock protein 70 genes in the ectoparasitoid Habrobracon hebetor (Hymenoptera: Braconidae)
Identifieur interne : 001D79 ( Main/Exploration ); précédent : 001D78; suivant : 001D80Transcript analysis and expression profiling of three heat shock protein 70 genes in the ectoparasitoid Habrobracon hebetor (Hymenoptera: Braconidae)
Auteurs : Hao-Liang Chen [République populaire de Chine, États-Unis] ; Hong-Yu Zhang [République populaire de Chine] ; James E. Throne [États-Unis] ; Kun Yan Zhu [États-Unis, République populaire de Chine]Source :
- Insect Science [ 1672-9609 ] ; 2014-08.
Abstract
Heat shock proteins (HSPs) are known as chaperones that help with folding of other proteins when cells are under environmental stresses. The upregulation of HSPs is essential for cold survival during insect diapause. The ectoparasitoid Habrobracon hebetor, a potential biological control agent, can enter reproductive diapause when reared at low temperature and short photoperiod. However, the expression of HSPs during diapause of H. hebetor has not been studied. In this study, we sequenced and characterized the full‐length complementary DNAs of three Hsp70 genes (HhHsp70I, HhHsp70II and HhHsp70III) from H. hebetor. Their deduced amino acid sequences showed more than 80% identities to their counterparts from other insect species. However, the multiple sequence alignment among the three deduced amino acid sequences of HhHsp70s showed only 46% identities. A phylogenetic analysis of the three HhHsp70s and all other known Hsp70 sequences from Hymenoptera clustered all the Hsp70s into four groups, and the three HhHsp70s were distributed into three different groups. Real‐time quantitative polymerase chain reaction analysis showed that the expression of the three HhHsp70 genes in H. hebetor reared at different conditions was quite different. HhHsp70I showed higher relative expression when H. hebetor were reared at 27.5°C than at two lower temperatures (17.5°C and 20°C) regardless of the photoperiod, whereas HhHsp70II showed higher expression when H. hebetor were reared at 20°C and 10 : 14 L : D than when reared at 17.5°C and either 16 : 8 L : D or 10 : 14 L : D. In contrast, HhHSP70III was expressed at similar levels regardless of the rearing conditions. These results may suggest functional differences among the three HhHsp70 genes in H. hebetor.
Url:
DOI: 10.1111/1744-7917.12032
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001B51
- to stream Istex, to step Curation: 001B51
- to stream Istex, to step Checkpoint: 000162
- to stream Main, to step Merge: 001D94
- to stream Main, to step Curation: 001D79
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Transcript analysis and expression profiling of three heat shock protein 70 genes in the ectoparasitoid Habrobracon hebetor (Hymenoptera: Braconidae)</title><author><name sortKey="Chen, Hao Iang" sort="Chen, Hao Iang" uniqKey="Chen H" first="Hao-Liang" last="Chen">Hao-Liang Chen</name></author><author><name sortKey="Zhang, Hong U" sort="Zhang, Hong U" uniqKey="Zhang H" first="Hong-Yu" last="Zhang">Hong-Yu Zhang</name></author><author><name sortKey="Throne, James E" sort="Throne, James E" uniqKey="Throne J" first="James E." last="Throne">James E. Throne</name></author><author><name sortKey="Zhu, Kun Yan" sort="Zhu, Kun Yan" uniqKey="Zhu K" first="Kun Yan" last="Zhu">Kun Yan Zhu</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:330EBA0025D708FDA575B20D4B1497210B9EEC47</idno><date when="2014" year="2014">2014</date><idno type="doi">10.1111/1744-7917.12032</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-3M13M6L1-W/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001B51</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001B51</idno><idno type="wicri:Area/Istex/Curation">001B51</idno><idno type="wicri:Area/Istex/Checkpoint">000162</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000162</idno><idno type="wicri:doubleKey">1672-9609:2014:Chen H:transcript:analysis:and</idno><idno type="wicri:Area/Main/Merge">001D94</idno><idno type="wicri:Area/Main/Curation">001D79</idno><idno type="wicri:Area/Main/Exploration">001D79</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Transcript analysis and expression profiling of three heat shock protein 70 genes in the ectoparasitoid Habrobracon hebetor (Hymenoptera: Braconidae)</title><author><name sortKey="Chen, Hao Iang" sort="Chen, Hao Iang" uniqKey="Chen H" first="Hao-Liang" last="Chen">Hao-Liang Chen</name><affiliation wicri:level="3"><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><wicri:orgArea>State Key Laboratory of Agricultural Microbiology, Institute of Urban and Horticultural Pests, Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University</wicri:orgArea></affiliation><affiliation wicri:level="3"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Plant Protection and Agro‐Products Safety, Anhui Academy of Agricultural Sciences, Hefei</wicri:regionArea><placeName><settlement type="city">Hefei</settlement><region type="province">Anhui</region></placeName></affiliation><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>USDA Agricultural Research Service, Center for Grain & Animal Health Research, Manhattan, KS</wicri:regionArea><wicri:noRegion>KS</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Entomology, Kansas State University, KS, Manhattan</wicri:regionArea><wicri:noRegion>Manhattan</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Hong U" sort="Zhang, Hong U" uniqKey="Zhang H" first="Hong-Yu" last="Zhang">Hong-Yu Zhang</name><affiliation wicri:level="3"><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><wicri:orgArea>State Key Laboratory of Agricultural Microbiology, Institute of Urban and Horticultural Pests, Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University</wicri:orgArea></affiliation><affiliation wicri:level="1"><country wicri:rule="url">République populaire de Chine</country></affiliation></author><author><name sortKey="Throne, James E" sort="Throne, James E" uniqKey="Throne J" first="James E." last="Throne">James E. Throne</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>USDA Agricultural Research Service, Center for Grain & Animal Health Research, KS, Manhattan</wicri:regionArea><wicri:noRegion>Manhattan</wicri:noRegion></affiliation></author><author><name sortKey="Zhu, Kun Yan" sort="Zhu, Kun Yan" uniqKey="Zhu K" first="Kun Yan" last="Zhu">Kun Yan Zhu</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Entomology, Kansas State University, KS, Manhattan</wicri:regionArea><wicri:noRegion>Manhattan</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">République populaire de Chine</country></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Insect Science</title><title level="j" type="alt">INSECT SCIENCE</title><idno type="ISSN">1672-9609</idno><idno type="eISSN">1744-7917</idno><imprint><biblScope unit="vol">21</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="415">415</biblScope><biblScope unit="page" to="428">428</biblScope><biblScope unit="page-count">14</biblScope><date type="published" when="2014-08">2014-08</date></imprint><idno type="ISSN">1672-9609</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1672-9609</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract"> Heat shock proteins (HSPs) are known as chaperones that help with folding of other proteins when cells are under environmental stresses. The upregulation of HSPs is essential for cold survival during insect diapause. The ectoparasitoid Habrobracon hebetor, a potential biological control agent, can enter reproductive diapause when reared at low temperature and short photoperiod. However, the expression of HSPs during diapause of H. hebetor has not been studied. In this study, we sequenced and characterized the full‐length complementary DNAs of three Hsp70 genes (HhHsp70I, HhHsp70II and HhHsp70III) from H. hebetor. Their deduced amino acid sequences showed more than 80% identities to their counterparts from other insect species. However, the multiple sequence alignment among the three deduced amino acid sequences of HhHsp70s showed only 46% identities. A phylogenetic analysis of the three HhHsp70s and all other known Hsp70 sequences from Hymenoptera clustered all the Hsp70s into four groups, and the three HhHsp70s were distributed into three different groups. Real‐time quantitative polymerase chain reaction analysis showed that the expression of the three HhHsp70 genes in H. hebetor reared at different conditions was quite different. HhHsp70I showed higher relative expression when H. hebetor were reared at 27.5°C than at two lower temperatures (17.5°C and 20°C) regardless of the photoperiod, whereas HhHsp70II showed higher expression when H. hebetor were reared at 20°C and 10 : 14 L : D than when reared at 17.5°C and either 16 : 8 L : D or 10 : 14 L : D. In contrast, HhHSP70III was expressed at similar levels regardless of the rearing conditions. These results may suggest functional differences among the three HhHsp70 genes in H. hebetor.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li><li>États-Unis</li></country><region><li>Anhui</li><li>Hubei</li></region><settlement><li>Hefei</li><li>Wuhan</li></settlement></list><tree><country name="République populaire de Chine"><region name="Hubei"><name sortKey="Chen, Hao Iang" sort="Chen, Hao Iang" uniqKey="Chen H" first="Hao-Liang" last="Chen">Hao-Liang Chen</name></region><name sortKey="Chen, Hao Iang" sort="Chen, Hao Iang" uniqKey="Chen H" first="Hao-Liang" last="Chen">Hao-Liang Chen</name><name sortKey="Zhang, Hong U" sort="Zhang, Hong U" uniqKey="Zhang H" first="Hong-Yu" last="Zhang">Hong-Yu Zhang</name><name sortKey="Zhang, Hong U" sort="Zhang, Hong U" uniqKey="Zhang H" first="Hong-Yu" last="Zhang">Hong-Yu Zhang</name><name sortKey="Zhu, Kun Yan" sort="Zhu, Kun Yan" uniqKey="Zhu K" first="Kun Yan" last="Zhu">Kun Yan Zhu</name></country><country name="États-Unis"><noRegion><name sortKey="Chen, Hao Iang" sort="Chen, Hao Iang" uniqKey="Chen H" first="Hao-Liang" last="Chen">Hao-Liang Chen</name></noRegion><name sortKey="Chen, Hao Iang" sort="Chen, Hao Iang" uniqKey="Chen H" first="Hao-Liang" last="Chen">Hao-Liang Chen</name><name sortKey="Throne, James E" sort="Throne, James E" uniqKey="Throne J" first="James E." last="Throne">James E. Throne</name><name sortKey="Zhu, Kun Yan" sort="Zhu, Kun Yan" uniqKey="Zhu K" first="Kun Yan" last="Zhu">Kun Yan Zhu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001D79 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001D79 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:330EBA0025D708FDA575B20D4B1497210B9EEC47
|texte= Transcript analysis and expression profiling of three heat shock protein 70 genes in the ectoparasitoid Habrobracon hebetor (Hymenoptera: Braconidae)
}}
| This area was generated with Dilib version V0.6.33. |  |